Week 5-9 of internship
I CLONED THE PLASMID !!!
Throughout March, I attempted to create my plasmids, repeating protocol over and over again (just changing its parameters, like temperature). And finally, I obtained the correct sequence of one plasmid out of the two that I need. I am so happy, OMG!
Actually, I had an idea to switch to another type of plasmid cloning - ligation using restriction enzymes - but it takes time to order the new primers with a restriction sequence, and this is expensive, bla bla bla. Well, I didn't get these reasons. In France, the reagents and samples do not get stuck somewhere between countries (if you know, you know), so three days is quite a short period of time to get what you need. Anyway we delayed this decision, attributing the cloning failures to the quite complex sequence of the plasmid itself: many repetitions in the sequence that could cause the plasmid duplication or prevent infusion itself
Despite all these difficulties, at least one plasmid worked out, which means I can move forward and start training to work with cells (I feel like a God: from DNA to cells). At the moment, I'm practicing on MEF cells (mouse embryonic fibroblast) because they are relatively easy to work with, and they grow quickly. But even with such undemanding cells, I managed to ruin them 😂 because I infected my baby cells with some bacteria (I didn't feel like a terrible mother of cells, but I felt a little guilty, they are too difficult to kill)))
I anticipate even more work and adventures in the lab. Stay tuned for more updates on my work in molecular biology!
#lab #MSc
I CLONED THE PLASMID !!!
Throughout March, I attempted to create my plasmids, repeating protocol over and over again (just changing its parameters, like temperature). And finally, I obtained the correct sequence of one plasmid out of the two that I need. I am so happy, OMG!
Actually, I had an idea to switch to another type of plasmid cloning - ligation using restriction enzymes - but it takes time to order the new primers with a restriction sequence, and this is expensive, bla bla bla. Well, I didn't get these reasons. In France, the reagents and samples do not get stuck somewhere between countries (if you know, you know), so three days is quite a short period of time to get what you need. Anyway we delayed this decision, attributing the cloning failures to the quite complex sequence of the plasmid itself: many repetitions in the sequence that could cause the plasmid duplication or prevent infusion itself
Despite all these difficulties, at least one plasmid worked out, which means I can move forward and start training to work with cells (I feel like a God: from DNA to cells). At the moment, I'm practicing on MEF cells (mouse embryonic fibroblast) because they are relatively easy to work with, and they grow quickly. But even with such undemanding cells, I managed to ruin them 😂 because I infected my baby cells with some bacteria (I didn't feel like a terrible mother of cells, but I felt a little guilty, they are too difficult to kill)))
I anticipate even more work and adventures in the lab. Stay tuned for more updates on my work in molecular biology!
#lab #MSc
Thermofisher
Ligature et digestion par enzymes de restriction | Thermo Fisher Scientific - FR
Présentation des produits d’enzymes de restriction et de modification de l’ADN à des fins de clonage par enzymes de restriction
Interesting news!
My friends, who created the wonderful SciXplore project, interviewed me about my life and work in France. The first part of the interview is already available 🙃 Stay tuned for my upcoming interview - you won't want to miss it!
https://www.tg-me.com/sciXplore/88
https://www.tg-me.com/sciXplore/88
https://www.tg-me.com/sciXplore/88
#France #MSc #lab
My friends, who created the wonderful SciXplore project, interviewed me about my life and work in France. The first part of the interview is already available 🙃 Stay tuned for my upcoming interview - you won't want to miss it!
https://www.tg-me.com/sciXplore/88
https://www.tg-me.com/sciXplore/88
https://www.tg-me.com/sciXplore/88
#France #MSc #lab
Bonjour à tous 🤍
Week 10 of internship was rather uneventful, with only one noteworthy thing : my second plasmid is also correct ! So I am done with it !! I gonna send you my protocol of plasmid cloning.
My work is completely changing, which I have already described above. I'm currently immersed in training related to fibroblast and virus manipulation. Exciting times lie ahead as I delve deeper into this new phase of my research journey
#lab #MSc
Week 10 of internship was rather uneventful, with only one noteworthy thing : my second plasmid is also correct ! So I am done with it !! I gonna send you my protocol of plasmid cloning.
My work is completely changing, which I have already described above. I'm currently immersed in training related to fibroblast and virus manipulation. Exciting times lie ahead as I delve deeper into this new phase of my research journey
#lab #MSc
Hey!
SciXplore released the second part and the third part of my interview. You will like it 🤍
#France #MSc #lab
SciXplore released the second part and the third part of my interview. You will like it 🤍
#France #MSc #lab
Telegram
sciXplore
Магистратура во Франции 🇫🇷
Université de Bordeaux: часть 2
❓Ты сказала что интервью с Университетом Бордо было чем-то необычным для тебя. Можешь подробнее рассказать?
Интервью было назначено на 17 апреля в 11:00. Я безумно волновалась, потому что в тот…
Université de Bordeaux: часть 2
❓Ты сказала что интервью с Университетом Бордо было чем-то необычным для тебя. Можешь подробнее рассказать?
Интервью было назначено на 17 апреля в 11:00. Я безумно волновалась, потому что в тот…
MEF cells counting with Malassez counting chamber
I need to count my baby MEF cells in order to take a certain amount (1,5 million) of them for more efficient and successful transfection with my plasmid
Before counting, MEF cells need to be prepared for the procedure. This typically involves harvesting the cells from a culture flask (where they live and grow) using trypsin. Once detached, the cells are centrifugated and then resuspended in a cell culture medium (DMEM in my case)
To count the cells, I load a small volume of the cell suspension into the Malassez counting chamber (Fig.1). The Malassez chamber is a specially designed slide with a grid etched onto the surface and a central chamber with a known volume (Fig.1). The grid on the Malassez chamber helps to visualize and count the cells. The cells are counted within several squares of the grid to ensure accuracy. I usually count them within several grids and then calculate the concentration of cells based on known volume of these grids.
After the calculation I just take a certain amount of the MEF cells for further viral transfection with my plasmids and the rest for growth and next sets of this manip
#lab #methods
I need to count my baby MEF cells in order to take a certain amount (1,5 million) of them for more efficient and successful transfection with my plasmid
Before counting, MEF cells need to be prepared for the procedure. This typically involves harvesting the cells from a culture flask (where they live and grow) using trypsin. Once detached, the cells are centrifugated and then resuspended in a cell culture medium (DMEM in my case)
To count the cells, I load a small volume of the cell suspension into the Malassez counting chamber (Fig.1). The Malassez chamber is a specially designed slide with a grid etched onto the surface and a central chamber with a known volume (Fig.1). The grid on the Malassez chamber helps to visualize and count the cells. The cells are counted within several squares of the grid to ensure accuracy. I usually count them within several grids and then calculate the concentration of cells based on known volume of these grids.
After the calculation I just take a certain amount of the MEF cells for further viral transfection with my plasmids and the rest for growth and next sets of this manip
#lab #methods
Look at this!
We are starting a new project - sciXbee 🚀
The sciXbee is a meeting in discord, where each participant will be able to communicate with the guest personally 🤍
At this meeting we will turn into bees that buzz about science and life in France with me as an invited speaker 🇫🇷 🥐🍷
🗓 Date: Sunday 14th April
🕑 Time: 2 p.m. (Moscow time)
🔭 Where: online in Discord
☕️ Language: Russian
SciXplore team and I will be glad to see you
Prepare the questions, we will buzz everything!
P.S. look at this marshmallow braaaain omg
💜🤍🩵💙
#I #France #MSc #lab #SciXbee
We are starting a new project - sciXbee 🚀
The sciXbee is a meeting in discord, where each participant will be able to communicate with the guest personally 🤍
At this meeting we will turn into bees that buzz about science and life in France with me as an invited speaker 🇫🇷 🥐🍷
🗓 Date: Sunday 14th April
🕑 Time: 2 p.m. (Moscow time)
🔭 Where: online in Discord
☕️ Language: Russian
SciXplore team and I will be glad to see you
Prepare the questions, we will buzz everything!
P.S. look at this marshmallow braaaain omg
💜🤍🩵💙
#I #France #MSc #lab #SciXbee
Telegram
sciXplore
Пожужжим? 🐝
Врываемся в вечер с радостной новостью: sciXplore запускает новый формат встреч — sciXbee!
На встречах мы с вами не просто на время станем пчелами и будем жужжать о науке, но и о жизни с приглашённым эксклюзивным гостем. Доступно со всех уголков…
Врываемся в вечер с радостной новостью: sciXplore запускает новый формат встреч — sciXbee!
На встречах мы с вами не просто на время станем пчелами и будем жужжать о науке, но и о жизни с приглашённым эксклюзивным гостем. Доступно со всех уголков…
Today was a meeting with Karl Deisseroth. He presented us the "Light-gated channels" lecture and told us about recent optogenetic discoveries made in his lab in Stanford University
I was pretty surprised that he made plenty of discoveries in such a relatively small piece of science (I am talking about just a certain small part of a single channel)
I like to attend lectures about different parts of neuroscience bcz such a versatile knowledge gives the form of science in my head, making it voluminous and understandable
P.S. Follow for more posts about optogenetics via the tag #optogenetics
#lab #people
I was pretty surprised that he made plenty of discoveries in such a relatively small piece of science (I am talking about just a certain small part of a single channel)
I like to attend lectures about different parts of neuroscience bcz such a versatile knowledge gives the form of science in my head, making it voluminous and understandable
P.S. Follow for more posts about optogenetics via the tag #optogenetics
#lab #people
Post about optogenetics in NeuroLife:
1. Introduction | link
2. Microbial opsins | link
3. Cool overview paper | link
#guides
1. Introduction | link
2. Microbial opsins | link
3. Cool overview paper | link
#guides
Telegram
NeuroLife 📎 in France
OPTOGENETICS
Optogenetics — is a biological technique that is used to alter the activity of neurons with light. Optogenetics involves the development of light-sensitive proteins (fast light-activated channels, pumps and enzymes), strategies for delivering…
Optogenetics — is a biological technique that is used to alter the activity of neurons with light. Optogenetics involves the development of light-sensitive proteins (fast light-activated channels, pumps and enzymes), strategies for delivering…